This book reflects the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Fluorescence lifetime approaches maximize contrast between different fluorophores for improved specificity of measurements, enabling characterization of intact tissue and disease diagnostics. "This highly recommended comprehensive volume is a good resource for investigators who wish to apply these techniques. It is a self-contained book in which the physics and the analytical methods are carefully worked out in detail. ? The discussions of fluorescence, the design and use of lifetime instrumentation, the various methods to analyze the data, and the biomedical applications are all current and well-illustrated."-Optics & Photonics News (OPN), October 2014"? a timely and comprehensive review of the state of the art by internationally leading experts in the field. It provides excellent coverage of the basic principles, as well as a thorough appraisal of the latest methods and applications. The book represents a major resource for researchers, students, and technologists."-Jem Hebden, Ph.D., Professor and Head, Department of Medical Physics and Bioengineering, University College London"This book provides comprehensive coverage on key aspects of fluorescence lifetime imaging, an emerging technique for life sciences and clinical diagnosis. The instrumentation and analysis sections include both well-known techniques and recent developments."-Dr. Qiyin Fang, Associate Professor of Engineering Physics, McMaster University